fibroblast culture medium fcm Search Results


fibroblast culture medium fcm  (Lonza)
90
Lonza fibroblast culture medium fcm
Human <t>fibroblast</t> seeding on the hypodermal side of d-NACs. (A) H&E staining of adherent human fibroblasts (arrows) on the acellular hypodermal side and, in some locations, adherent to the epidermal side of d-NACs after 7 days of static culture (scale bar = 200 μm). (A’) Higher magnification of the H&E-stained section highlighting adherent fibroblasts forming several cell layers on the hypodermal side of the scaffold (scale bar = 100 μm). (B) Live/dead staining of seeded fibroblasts shows a high viability on day 7 of the culture on the scaffold (living cells = green and dead cells = red) (scale bar = 500 μm). (C) Cell viability of the seeded dermis: ECM- red and control wells- blue. The results are expressed as the mean cell viability. Error bars: SD; ns = not significant. (D) A PrestoBlue cell viability assay realized on seeded d-NACs (red, n = 3) and control culture wells (blue, n = 3) attests the biocompatibility of the produced scaffolds by the increase in metabolic activity during the 7 days of culture. The results are expressed as the mean fluorescence intensity. Error bars: SD; ** p < 0.01; ns = not significant.
Fibroblast Culture Medium Fcm, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fibroblast+culture+medium+fcm/pmc10902434-153-5-13?v=Lonza
Average 90 stars, based on 1 article reviews
fibroblast culture medium fcm - by Bioz Stars, 2026-07
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Human fibroblast seeding on the hypodermal side of d-NACs. (A) H&E staining of adherent human fibroblasts (arrows) on the acellular hypodermal side and, in some locations, adherent to the epidermal side of d-NACs after 7 days of static culture (scale bar = 200 μm). (A’) Higher magnification of the H&E-stained section highlighting adherent fibroblasts forming several cell layers on the hypodermal side of the scaffold (scale bar = 100 μm). (B) Live/dead staining of seeded fibroblasts shows a high viability on day 7 of the culture on the scaffold (living cells = green and dead cells = red) (scale bar = 500 μm). (C) Cell viability of the seeded dermis: ECM- red and control wells- blue. The results are expressed as the mean cell viability. Error bars: SD; ns = not significant. (D) A PrestoBlue cell viability assay realized on seeded d-NACs (red, n = 3) and control culture wells (blue, n = 3) attests the biocompatibility of the produced scaffolds by the increase in metabolic activity during the 7 days of culture. The results are expressed as the mean fluorescence intensity. Error bars: SD; ** p < 0.01; ns = not significant.

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Reconstruction of the human nipple–areolar complex: a tissue engineering approach

doi: 10.3389/fbioe.2023.1295075

Figure Lengend Snippet: Human fibroblast seeding on the hypodermal side of d-NACs. (A) H&E staining of adherent human fibroblasts (arrows) on the acellular hypodermal side and, in some locations, adherent to the epidermal side of d-NACs after 7 days of static culture (scale bar = 200 μm). (A’) Higher magnification of the H&E-stained section highlighting adherent fibroblasts forming several cell layers on the hypodermal side of the scaffold (scale bar = 100 μm). (B) Live/dead staining of seeded fibroblasts shows a high viability on day 7 of the culture on the scaffold (living cells = green and dead cells = red) (scale bar = 500 μm). (C) Cell viability of the seeded dermis: ECM- red and control wells- blue. The results are expressed as the mean cell viability. Error bars: SD; ns = not significant. (D) A PrestoBlue cell viability assay realized on seeded d-NACs (red, n = 3) and control culture wells (blue, n = 3) attests the biocompatibility of the produced scaffolds by the increase in metabolic activity during the 7 days of culture. The results are expressed as the mean fluorescence intensity. Error bars: SD; ** p < 0.01; ns = not significant.

Article Snippet: HFs were mixed in a fibroblast culture medium (FCM) consisting of DMEM (BE12-604F, Lonza, Westburg, Netherlands) containing 10% FBS (10270–106, Thermo Fisher Scientific), 1% L-glutamine (BE17-605E, Lonza, Westburg, Netherlands), and 1% P/S.

Techniques: Staining, Control, Viability Assay, Produced, Activity Assay, Fluorescence

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Reconstruction of the human nipple–areolar complex: a tissue engineering approach

doi: 10.3389/fbioe.2023.1295075

Figure Lengend Snippet:

Article Snippet: HFs were mixed in a fibroblast culture medium (FCM) consisting of DMEM (BE12-604F, Lonza, Westburg, Netherlands) containing 10% FBS (10270–106, Thermo Fisher Scientific), 1% L-glutamine (BE17-605E, Lonza, Westburg, Netherlands), and 1% P/S.

Techniques: Immunofluorescence, Immunohistochemistry, Immunopeptidomics, Saline, Standard Deviation